Redesigning the NEDD8 Pathway with a Bacterial Genetic Screen for Ubiquitin-Like Molecule Transfer

详细信息	查看全文 | 推荐本文 |

• 作者：Gurkan Guntas ; Brian Kuhlman ; ^{bkuhlman@email.unc.edu}
• 关键词：NEDD8 ; ubiquitin ; Ubc12 ; APPBP1&ndash ; UBA3
• 刊名：Journal of Molecular Biology
• 出版年：2012
• 期刊代码：102_00222836
• 类别：imm
• 出版时间：4 May, 2012
• 卷：418
• 期：3-4
• 页码：161-166
• 文件大小：595 K

摘要

Pathways of ubiquitin-like (UBL) molecule transfer regulate a myriad of cellular cascades. Here, we report a high-throughput assay that correlates catalytic human NEDD8 transfer to bacterial survival. The assay was utilized to screen mutant NEDD8 and NAE (NEDD8-activating enzyme) libraries to engineer a more stable NEDD8 and redesign the NEDD8-NAE interface. This approach will be useful in understanding the specificities underlying UBL pathways.