Forms of phosphorus in bacteria and fungi isolated from two Australian soils

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• 作者：E.K. Bü ; nemann ; R.J. Smernik ; A.L. Doolette ; P. Marschner ; R. Stonor ; S.A. Wakelin ; A.M. McNeill
• 关键词：Bacteria ; Fungi ; Organic phosphorus ; ³¹P solution NMR ; Spectral deconvolution
• 刊名：Soil Biology & Biochemistry
• 出版年：2008
• 期刊代码：96_00380717
• 类别：abs
• 出版时间：July 2008
• 卷：40
• 期：7
• 页码：1908-1915
• 文件大小：710 K

摘要

To understand the origin of organic and condensed forms of phosphorus (P) in soils, detailed information about P forms in microorganisms is required. We isolated 7 bacteria and 8 fungi from two Australian soils and analyzed the P forms in their pure cultures by extraction with NaOH-EDTA followed by ³¹P solution nuclear magnetic (NMR) spectroscopy. The bacteria belonged to the actinobacteria and the fungi to the ascomycota, as determined by rDNA sequencing. The proportions of broad forms of P were significantly different between the bacterial and fungal isolates (analysis of similarities, p = 0.001). Ortho-, pyro- and polyphosphate were present in higher proportions in fungi, while monoester and diester P were present in higher proportions in bacteria. Spectral deconvolution of the monoester region revealed 15 distinct resonances. The three major ones, which were identified by spiking experiments as glycerol 1-phosphate, glycerol 2-phosphate and adenosine-5′-monophosphate (AMP), comprised 56–74%of P in the monoester region. Ordination by principal component analysis and testing for treatment effects using analysis of similarities showed significant separation of P distribution in the monoester region between bacterial and fungal isolates (p = 0.007). However, neither group of microorganisms had a specific single P form which might be considered characteristic. As such, it may be difficult to distinguish soil P from bacterial or fungal origins, with the possible exception of a predominantly fungal origin of pyro- and polyphosphate. The identification of three major resonances in the monoester region of microorganisms is important, since the same resonances are found in ³¹P NMR spectra of soil extracts.