Key strongylid nematodes of animals 鈥?Impact of next-generation transcriptomics on systems biology and biotechnology
- 作者：Cinzia Cantacessi ; Bronwyn E. Campbell ; Robin B. Gasser ; robinbg@unimelb.edu.au
- 关键词：Transcriptomics ; Strongylid nematodes ; Bioinformatics ; Vaccine targets ; Drug candidates ; Ancylostoma-secreted proteins ; Genomics ; Next-generation sequencing
- 刊名：Biotechnology Advances
- 出版年：2012
- 期刊代码：22_07349750
- 类别：abs
- 出版时间：May-June, 2012
- 卷：30
- 期：3
- 页码：469-488
- 文件大小：729 K
摘要
The advent and integration of high-throughput 'omic technologies (e.g., genomics, transcriptomics, proteomics and metabolomics) are becoming instrumental to assist fundamental explorations of the systems biology of organisms. In particular, these technologies now provide unique opportunities for global, molecular investigations of parasites. For example, studies of the transcriptomes (all transcripts in an organism, tissue or cell) of different species and/or developmental stages of parasitic nematodes provide insights into aspects of gene expression, regulation and function, which is a major step to understanding their biology. The purpose of this article was to review salient aspects of the systematics and biology of selected species of parasitic nematodes (particularly key species of the order Strongylida) of socio-economic importance, to describe conventional and advanced sequencing technologies and bioinformatic tools for large-scale investigations of the transcriptomes of these parasites and to highlight the prospects and implications of these explorations for developing novel methods of parasite intervention.