A comparison of standard cultural methods for the detection of foodborne Salmonella species including three new chromogenic plating media
- 作者：Vanessa Schö ; nenbrü ; cher ; Edward T. Mallinson ; Michael Bü ; lte
- 关键词：Salmonella ; ISO 6579 ; Chromogenic media ; Ground meat
- 刊名：International Journal of Food Microbiology
- 出版年：2008
- 期刊代码：63_01681605
- 类别：abs
- 出版时间：31 March 2008
- 卷：123
- 期：1-2
- 页码：61-66
- 文件大小：284 K
摘要
In this study the draft of the horizontal method for the detection of Salmonella species from human food and animal feed (ISO 6579:2002) was compared to the European gold standard (DIN EN 12824:1998), including the three new chromogenic plating media AES Salmonella Agar Plate (ASAP), Oxoid Salmonella Chromogen Media (OSCM) and Miller–Mallinson agar (MM). First the growth and appearance of 36 bacterial type strains (Salmonella and other 21 species) on ASAP, OSCM and MM were compared to those on the three traditional agars Brilliant Green Agar according to Edel and Kampelmacher (BGA), Xylose Lysine Deoxycholate Agar (XLD) and Xylose Lysine Tergitol 4 Agar (XLT4). Only on MM agar, did all of 36 tested type strains produce typical colonies, especially strains of S. Senftenberg, Salmonella arizonae, S. Dublin and S. Derby. Artificial inoculation experiments using raw pork ground meat (n = 92) were subsequently conducted. A shortened incubation time of 24 h in RVS broth yielded a Salmonella species recovery of 100%from spiked meat samples. Finally, 286 naturally contaminated raw porcine and bovine minced meat samples and raw poultry meat samples were investigated. Forty-three strains from a total of 39 Salmonella-positive samples were found.