The anti-inflammatory and anti-apoptotic effects of nesfatin-1 in the traumatic rat brain
- 作者：Chong-Hui Tang ; Xiao-Jun Fu ; chonghuitang@126.com ; Xin-Long Xu ; Xiao-Jie Wei ; Hong-Song Pan
- 关键词：Traumatic brain injury ; Nesfatin-1 ; Inflammation ; Neuronal cell apoptosis
- 刊名：Peptides
- 出版年：2012
- 期刊代码：59_01969781
- 类别：neu
- 出版时间：July, 2012
- 卷：36
- 期：1
- 页码：39-45
- 文件大小：2133 K
摘要
Nesfatin-1 has been demonstrated to possess anti-inflammatory and anti-apoptotic effects in the rat brain with subarachnoid hemorrhage. The study was designed to investigate the influence of nesfatin-1 on inflammatory responses and neuronal cell apoptosis after traumatic brain injury. Wistar rats were subjected to 5, 10 or 20 渭g/kg of nesfatin-1 at designed time points (0.5, 2, 4 or 8 h after head trauma) intraperitoneally. Rats were sacrificed at hours 2, 6 and 12, as well as day 1, 2, 3 and 5 after head trauma. The administration of 10 or 20 渭g/kg of nesfatin-1 at hour 0.5 after head trauma could significantly suppress gene expressions of nuclear factor kappa-B, lessen concentrations of tumor necrosis factor-alpha, interleukin-1beta and interleukin-6, diminish caspase-3 activity as well as reduce number of apoptotic neuronal cells in traumatic rat brain tissues (P < 0.05), but the administration of 5 渭g/kg of nesfatin-1 not (P > 0.05). Moreover, 20 渭g/kg nesfatin-1 also significantly suppressed the inflammation and neuronal cell apoptosis when applied 2, 4 or 8 h after head trauma. However, a clear concentration-response or time-response relationship was not found. These findings suggest that nesfatin-1 may inhibit nuclear factor kappa-B-dependent inflammatory responses, and lessen caspase-3-mediated neuronal cell apoptosis after traumatic brain injury in rats.