Chromosomal aberrations in cell lines derived from thyroid tumors spontaneously developed in TRβ^PV/PV mice

详细信息	查看全文 | 推荐本文 |

• 作者：Drazen B. Zimonjic ; Yasuhito Kato ; Hao Ying ; Nicholas C. Popescu and Sheue-Yann Cheng
• 刊名：Cancer Genetics and Cytogenetics
• 出版年：2005
• 期刊代码：43_01654608
• 类别：med
• 出版时间：2005
• 卷：161
• 期：2
• 页码：104-109
• 文件大小：299 K

摘要

The etiology and genetic alterations of follicular thyroid carcinoma are not well understood. By targeting a mutation (PV) into the thyroid hormone receptor β gene (TRβPV mouse), we created a knock-in mutant TRβ^PV/PV mouse that spontaneously develop follicular thyroid carcinoma with progression to metastasis similar to human follicular thyroid carcinoma. This mouse model provides a valuable tool to ascertain the nature and the extent of genomic rearrangements that occur during carcinogenesis of the thyroid. Spectral karyotyping analysis (SKY) of seven cell lines derived from thyroid tumors developed in TRβ^PV/PV mice showed that all of them had abnormal karyotypes, with chromosome number ranging from near-diploid (39–42 chromosomes) to hypotetraploid (63–79 chromosomes). These seven cell lines also exhibited a variety of structural chromosomal aberrations, including common recurrent translocations and deletions. This SKY analysis shows that the development and progression of follicular thyroid carcinoma in knock-in TRβ^PV/PV mutant mice comprise recurrent structural and numerical genomic changes, some of which mimic those described in human thyroid cancer.