Novel fluorescent proteins for high-content screening

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• 作者：Michael Wolff ; Joerg Wiedenmann ; G. Ulrich Nienhaus ; Martin Valler ; Ralf Heilker
• 刊名：Drug Discovery Today
• 出版年：2006
• 期刊代码：29_13596446
• 类别：imm
• 出版时间：December 2006
• 卷：11
• 期：23-24
• 页码：1054-1060
• 文件大小：564 K

摘要

The development of fast microscopic imaging devices has enabled the application of automated fluorescence microscopy to pharmaceutical high-throughput drug-discovery assays, referred to as high-content screening (HCS). Initially, green fluorescent protein and its derivatives from Aequorea Victoria, and later anthozoan fluorescent proteins (FPs) have become potent tools as live-cell markers in HCS assays. We illustrate the broad applicability of classic and novel FPs to drug-discovery assays, giving example applications of the use of FPs in multiplexed imaging as fluorescent timers, photosensitizers and pulse-chase labels, and for robotically integrated compound testing.