Efficacy of aerosolized hydrogen peroxide-based sanitizer on the reduction of pathogenic bacteria on a stainless steel surface

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• 作者：Na-Young Choi^a ; Seung-Youb Baek^a ; Jae-Hyun Yoon^a ; Mi-Ran Choi^a ; Dong-Hyun Kang^b ; Sun-Young Lee^a ; ^{nina6026@gmail.com} ; ^{nina6026@cau.ac.kr}
• 关键词：Foodborne pathogens ; Aerosolization ; Sanitizer ; Attached cells ; Stainless steel
• 刊名：Food Control
• 出版年：2012
• 期刊代码：49_09567135
• 类别：abs
• 出版时间：September, 2012
• 卷：27
• 期：1
• 页码：57-63
• 文件大小：347 K

摘要

This study was conducted to investigate the survival rates of Escherichia coli O157:H7, Salmonella Typhimurium and Listeria monocytogenes on stainless steel surfaces at 25聽掳C and an approximately 70%relative humidity, as well as the efficacy of two concentrations of an aerosolized hydrogen peroxide-based sanitizer on the reduction of the bacterial populations on stainless steel surfaces. Three different strains of bacteria, E.聽coli O157:H7, L.聽monocytogenes, and S. Typhimurium, were suspended in phosphate buffer with saline (PBS) or M9 minimal broth, inoculated onto a stainless steel surface and stored at room temperature for seven days or were treated with an aerosolized hydrogen peroxide-based sanitizer at concentrations of 0.25%and 0.5%for 0, 15, 30 and 60聽min. Initial bacteria levels before storage聽were 10^5-6聽CFU/coupon for E.聽coli O157:H7 and S. Typhimurium and 10^4-6聽CFU/coupon for L.聽monocytogenes. The populations decreased as the storage time increased. However, the rates of reduction before and after storage varied greatly depending on the bacterial strain. Generally, levels of E.聽coli O157:H7 were highly reduced during storage compared to those of S. Typhimurium and L.聽monocytogenes. The survival rate of the pathogens on the stainless steel surface was higher when M9 minimal broth was used than with PBS. Treatments with aerosolized hydrogen peroxide-based sanitizer were effective at reducing the levels of pathogens on the stainless steel surfaces. Levels of the three pathogens were below the detection limit (1聽log聽CFU/ml) after 60聽min with the 0.25%concentration. When the 0.5%sanitizer was used, levels of E.聽coli O157:H7 were below the detection limit after 15聽min, and levels of L.聽monocytogenes and S. Typhimurium were reduced to below the detection limit after 30 and 60聽min, respectively. These results suggest that the survival rates of pathogens on surfaces such as stainless steel vary depending on the bacterial strains and conditions, and that aerosolized hydrogen peroxide-based sanitizer are effective to remove these pathogens from surfaces.