摘要
NLRP3 inflammasome plays a critical role in the activation of caspase-1 and maturation of IL-1尾. However, the specific cis- and trans-regulatory elements that determine the extent of NLRP3 expression are not well defined. In this study, we found NLRP3 expression was induced by TLR agonists in murine macrophages in a NF-魏B dependent manner. Furthermore, the corresponding NF-魏B binding sites (nt 鈭?303 to 鈭?292 and 鈭?238 to 鈭?228) were identified in the NLRP3 promoter. Finally, EMSA and ChIP assays demonstrated LPS-induced NF-魏B binding to the NLRP3 promoter. Therefore, out results delineated the molecular mechanisms involved in TLR-induced transcriptional regulation of NLRP3.