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• 作者：Kimiyasu Isobe ; Keigo Tokuta ; Shouko Nagasawa ; Akira Matsuura ; Takehiko Sakaguchi ; Norio Wakao
• 关键词：N^α ; -Z-l-lysine ; N^α ; -Z-d-lysine ; N^α ; -Z-l-aminoadipate δ ; -semialdehyde ; N^α ; -Z-d-aminoadipate δ ; -semialdehyde ; N^
• 刊名：Enzyme and Microbial Technology
• 出版年：2007
• 期刊代码：26_01410229
• 类别：ce
• 出版时间：5 February 2007
• 卷：40
• 期：3
• 页码：433-436
• 文件大小：206 K

摘要

New biochemical methods for the production of N^α-benzyloxycarbonyl-l-aminoadipate δ-semialdehyde (N^α-Z-l-AASA), N^α-Z-d-AASA, N^α-Z-l-aminoadipic acid (N^α-Z-l-AAA), N^α-Z-d-AAA were developed using cells of Rhodococcus sp. AIU Z-35-1. When the cells harvested after 1 day of cultivation were incubated with 100 mM N^α-Z-l-lysine at pH 5.0 for 1 day at 30 °C or at pH 7.0 for 2 days at 10 °C, more than 95 mM N^α-Z-l-AASA was produced. Using the cells harvested after 3 days of cultivation, N^α-Z-l-AAA was efficiently produced by incubating at pH 7.0 for 4 days at 30 °C. Similar conversion yields of N^α-Z-d-AASA and N^α-Z-d-AAA were also obtained under the same conditions. Thus, the microbial methods proposed here were superior to the chemical and other biochemical methods in simplicity, reaction rate, and yield.