Co-localization of hippocampal cholinergic neurostimulating peptide precursor with collapsin response mediator protein-2 at presynaptic terminals in hippocampus

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• 作者：Daisuke Kato^a ; Shigehisa Mitake^a ; Masayuki Mizuno^a ; Tetsuko Kanamori^a ; Tatsuo Suzuki^b ; Kosei Ojika^a ; Noriyuki Matsukawa^a ; ^{norim@med.nagoya-cu.ac.jp}
• 关键词：HCNP ; hippocampal cholinergic neurostimulating peptide ; HCNP-pp ; hippocampal cholinergic neurostimulating peptide precursor protein ; PBP(PEBP) ; phosphatidylethanolamine binding protein ; RKIP ; raf-1 kinase inhibitory protein ; CRMP-2 ; collapsin response med
• 刊名：Neuroscience Letters
• 出版年：2012
• 期刊代码：52_03043940
• 类别：neu
• 出版时间：31 May, 2012
• 卷：517
• 期：2
• 页码：92-97
• 文件大小：897 K

摘要

Hippocampal cholinergic neurostimulating peptide (HCNP) induces the synthesis of acetylcholine in medial septal nucleus in vitro and in vivo. HCNP precursor protein (HCNP-pp) is a multifunctional protein that participates in a number of signaling pathways, including MAPK/extracellular signal and G-protein-coupled receptor kinase 2. We recently demonstrated that the amount of collapsin response mediator protein-2 (CRMP-2) is increased in hippocampus of HCNP-pp transgenic mice. To clarify the interaction between HCNP/HCNP-pp and CRMP-2 and its role in synaptic function, we investigated whether HCNP-pp is localized to the synapse and if it affects protein expression. Here, we demonstrate that HCNP-pp co-localizes with CRMP-2 at presynaptic terminals. Furthermore, HCNP-pp overexpression increases synaptophysin levels. These findings suggest that HCNP-pp, in association with CRMP-2, plays an important role in presynaptic function in the hippocampus.