Cloning and characterisation of a hyp gene cluster in the filamentous cyanobacterium Nostoc sp. strain PCC 73102

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• 作者：Hansel ; Alfred ; Axelsson ; Rikard ; Lindberg ; Pia ; Troshina ; Olga Y. ; Wü ; nschiers ; Rö ; bbe ; et. al.
• 关键词：Cyanobacterium ; Nostoc ; Uptake hydrogenase ; Hydrogenase accessory hyp gene ; Transcription ; Reverse transcription polymerase chain reaction
• 刊名：FEMS Microbiology Letters
• 出版年：2001
• 期刊代码：34_03781097
• 类别：imm
• 出版时间：July 10, 2001
• 卷：201
• 期：1
• 页码：59-64
• 文件大小：338 K

摘要

Maturation of [NiFe]-hydrogenases requires the action of several groups of accessory genes. Homologues of one group of these genes, the so-called hyp genes, putatively encoding proteins participating in the formation of an active uptake hydrogenase in the filamentous, heterocyst-forming cyanobacterium Nostoc PCC 73102, were cloned. The cluster, consisting of hypF, hypC, hypD, hypE, hypA, and hypB, is located 3.8 kb upstream from the uptake hydrogenase-encoding hupSL. Gene expression analyses show that these hyp genes are, like hupL, transcribed under N₂-fixing but not under non-N₂-fixing growth conditions. Furthermore, the six hyp genes are transcribed together with an open reading frame upstream of hypF, as a single mRNA. Analysis of the DNA region upstream of the experimentally determined transcriptional start site revealed putative −10 and −35 sequence elements and putative binding sites for the global nitrogen regulator NtcA.