DNA was extracted from peripheral blood of 1060 individuals (391 patients diagnosed with NSCLC and a control group of 669 individuals without cancer). The characterization of IL-4 鈭?#xA0;590C/T genotypes was performed by PCR-RFLP (BsmFI).
The 鈭?em>590C/T polymorphism genotypes were classified as low (CC) and high expression (TT).
The frequencies obtained for the CC and TT genotypes were 90.1%and 9.9%, respectively, in the control group and 92.9%and 7.1%, respectively, in the case group. The analysis of the TT and CC genotype frequencies in the two groups showed a statistically significant difference in its distribution, indicating a protection of 80%for the development of NSCLC, type epidermoid in individuals with the TT genotype when compared with individuals with CC genotype (P = 0.024, OR = 0.221: 95%CI = 0.053-0.928).
We present for the first time that increased expression of IL-4 associated with the TT genotype may contribute to immune surveillance during NSCLC development.