Study of animal species (human, dog and cat) identification using a multiplex single-base primer extension reaction in the cytochrome b gene
- 作者：Shin-ichi Nakaki ; Daiki Hino ; Miki Miyoshi ; Hideki Nakayama ; Hiroyuki Moriyoshi ; Toshio Morikawa ; Koji Itohara
- 关键词：Mitochondrial DNA ; Cytochrome b gene ; Animal species identification ; Multiplex single-base primer extension reaction ; Hair shafts ; Forensic casework sample
- 刊名：Forensic Science International
- 出版年：2007
- 期刊代码：104_03790738
- 类别：med
- 出版时间：20 December 2007
- 卷：173
- 期：2-3
- 页码：97-102
- 文件大小：883 K
摘要
We developed a simple method for animal species identification of humans, dogs and cats, using a multiplex single-base primer extension reaction in the cytochrome b gene. Using this method, three points of a single nucleotide in the cytochrome b gene were examined in these species using primers of different lengths. Our method was found to be able to successfully identify humans (26 samples), dogs (21 samples) and cats (9 samples), and no differences were found among the samples from each animal species in this study. The amount of template DNA required was over 0.01 ng for humans and dogs, and over 0.1 ng for cats. The present method was able to identify animal species from hair shaft (2 cm) and forensic casework samples (blood stains and hair shafts), and is thus a useful tool for animal species (human, dog and cat) identification in forensic science.