Efficacy of 3M鈩?Petrifilm鈩?aerobic count plates for enumerating Bacillus sporothermodurans and Geobacillus stearothermophilus in UHT milk

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• 作者：Rosa M. Casillas-Buenrostro^a ; Norma L. Heredia^a ; DeAnn L. Benesh^b ; Santos Garcí ; a^a ; ^{santos@microbiosymas.com}
• 刊名：International Dairy Journal
• 出版年：2012
• 期刊代码：59_09586946
• 类别：abs
• 出版时间：August, 2012
• 卷：25
• 期：2
• 页码：147-149
• 文件大小：116 K

摘要

The effectiveness of the 3M鈩?Petrifilm鈩?aerobic count plate for determining the amount of Bacillus sporothermodurans and Geobacillus stearothermophilus in ultra high temperature milk was determined and its efficacy was compared with the pour-plate agar and one-streak nutrient agar methods. Tubes containing milk were inoculated with spore suspensions, heat shocked and incubated. Aliquots were collected after 0, 24, 48, and 72 h of incubation, inoculated using the three different plate methods, and incubated at 55 掳C. B.聽sporothermodurans was detected at 9 h on the Petrifilm plate, compared with 18 h for both the pour plate and one-streak methods. Depending on the strain, G.聽stearothermophilus was detected between 6 and 12 h by the Petrifilm plate method and between 9 and 12 h by the other methods. The Petrifilm plates gave reproducible results compared with the traditional methods and are compatible with industrial requirements for milk quality control.