Identification of a point mutation associated with pyrethroid resistance in the para-type sodium channel of Triatoma infestans, a vector of Chagas鈥?disease

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• 作者：Jé ; sica Fabro^a ; Marcos Sterkel^a ; Natalia Capriotti^a ; Gast&oacute ; n Mougabure-Cueto^b ; M&oacute ; nica Germano^b ; Rolando Rivera-Pomar^a ; ^c ; Sheila Ons^a ; ^{sheila.ons@presi.unlp.edu.ar}
• 关键词：kdr ; knockdown resistance ; PASA ; PCR amplification of specific alleles ; R ; resistant population ; RR ; resistance ratio ; S ; sensitive strain ; WGS ; whole genome sequence
• 刊名：Infection ; Genetics and Evolution
• 出版年：2012
• 期刊代码：119_15671348
• 类别：med
• 出版时间：March, 2012
• 卷：12
• 期：2
• 页码：487-491
• 文件大小：626 K

摘要

The voltage-gated sodium channel is the target site of pyrethroid insecticides. Point mutations in the domain II region of the channel have been implicated in pyrethroid resistance of several insect species. We identified the sequence of domain II from the para sodium channel in Rhodnius prolixus, a vector of Chagas鈥?disease. With this information, we cloned and sequenced the domain II of the sodium channel from the other main Chagas鈥?disease vector: Triatoma infestans. We also identified the presence of a resistance-conferring mutation (L1014F) in a pyrethroid-resistant population of T. infestans from Argentina, and present a PCR-based method to detect this mutation in individuals from field populations. These findings have important implications for the implementation of strategies for resistance management, and for the rational design of campaigns for the control of Chagas鈥?disease transmission.