Microfluidic technology: An economical and versatile approach for the synthesis of O-(2-[¹⁸F]fluoroethyl)-l-tyrosine ([¹⁸F]FET)

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• 作者：Vincent Bouvet ; Melinda Wuest ; Pui-Hang Tam ; Monica Wang ; Frank Wuest ; ^{wuest@ualberta.ca}
• 关键词：[18F]Fluoroethyltyrosine ([18F]FET) ; Microfluidic technology ; Positron emission tomography (PET) ; Small animal PET imaging
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2012
• 期刊代码：10_0960894x
• 类别：ch
• 出版时间：15 March, 2012
• 卷：22
• 期：6
• 页码：2291-2295
• 文件大小：630 K

摘要

A new synthesis of O-(2-[¹⁸F]fluoroethyl)-l-tyrosine [¹⁸F]FET was developed using a NanoTek庐 microfluidic synthesis system (Advion BioSciences, Inc.). Optimal reaction conditions were studied through screening different reaction parameters like temperature, flow rate, reaction time, concentration of the labeling precursor, and the applied volume ratio between the labeling precursor and [¹⁸F]fluoride. [¹⁸F]FET was obtained after HPLC purification with 50%decay-corrected radiochemical yield starting from as little as 40 渭g of labeling precursor. Small animal PET studies in EMT-6 tumor bearing mice showed radioactivity accumulation in the tumor (SUV_60min 1.21 卤 0.2) resulting in an slightly increasing tumor-to-muscle ratio over time.