An RNA biding protein, Y14 interacts with and modulates STAT3 activation
- 作者：Norihiko Ohbayashi ; Naohisa Taira ; Shiho Kawakami ; Sumihito Togi ; Noriko Sato ; Osamu Ikeda ; Shinya Kamitani ; Ryuta Muromoto ; Yuichi Sekine ; Tadashi Matsuda
- 关键词：Cytokine ; STAT3 ; Y14 ; RNA-binding protein ; Gene expression ; Signal transduction
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2008
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：1 August 2008
- 卷：372
- 期：3
- 页码：475-479
- 文件大小：969 K
摘要
Signal transducer and activator of transcription 3 (STAT3), which mediates biological actions in many physiological processes, is activated by cytokines and growth factors via specific tyrosine-phosphorylation, dimerization, and nuclear translocation. To clarify the molecular mechanisms underlying the regulation of STAT3 activation, we performed yeast two-hybrid screening. We identified Y14, an RNA-binding protein, as a novel STAT3 binding partner. Y14 bound to STAT3 through the C-terminal region of STAT3 in vivo. Importantly, small-interfering RNA-mediated reduction of endogenous Y14 expression decreased IL-6-induced tyrosine-phosphorylation, nuclear accumulation, and DNA-binding activity of STAT3, as well as IL-6/STAT3-dependent gene expression. These results indicate that Y14 interacts with STAT3 and regulates the transcriptional activation of STAT3 by influencing the tyrosine-phosphorylation of STAT3.