Cell death in NF-κB-dependent tumour cell lines as a result of NF-κB trapping by linker-modified hairpin decoy oligonucleotide
- 作者：Christelle Laguillier ; Ali Tadlaoui Hbibi ; Fanny Baran-Marszak ; Valeri Metelev ; An Cao ; Florence Cymbalista ; Jr. ; Alexei Bogdanov ; Remi Fagard
- 关键词：Decoy oligonucleotide ; NF-κ ; B ; Tumour cell lines ; Cell death ; Linker-modification
- 刊名：FEBS Letters
- 出版年：2007
- 期刊代码：70_00145793
- 类别：bio
- 出版时间：20 March 2007
- 卷：581
- 期：6
- 页码：1143-1150
- 文件大小：805 K
摘要
The transcription factor NF-κB is frequently activated in cancer, and is therefore a valuable target for cancer therapy. Decoy oligodeoxynucleotides (ODNs) inhibit NF-κB by preventing its binding to the promoter region of target genes. Few studies have used NF-κB-targeting with ODNs in cancer. Using a hairpin NF-κB-decoy ODN we found that it induced growth inhibition and cell death in NF-κB-dependent tumour cell lines. The ODN colocalized with the p50 subunit of NF-κB in cells and directly interacted with it in nuclear extracts. In TNFα-treated cells the ODN and the p50 subunit were found in the cytoplasm suggesting that the complex did not translocate to the nucleus. Transcriptional activity of NF-κB was efficiently inhibited by the ODN, whereas a scrambled ODN was without effect on transcription. Thus, ODN-mediated inhibition of NF-κB can efficiently promote cell death in cancer cells providing a potentially powerful approach to tumour growth inhibition.