Antifibrotic effect via the regulation of transcription factor Sp1 in lung fibrosis
- 作者：Yoon-Seup Kum ; Kyung-Hyun Kim ; Tae-In Park ; In-Soo Suh ; Hoon-Kyu Oh ; Chang-Ho Cho ; Jae-Bok Park ; Young-Chae Chang ; Ji-Hyun Park ; Kwang-Gil Lee ; Kwan-Kyu Park
- 关键词：TGF-β ; 1 ; Transcription factor Sp1 ; Decoy oligodeoxynucleotide ; Lung fibrosis
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2007
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：16 November 2007
- 卷：363
- 期：2
- 页码：368-374
- 文件大小：4606 K
摘要
The aim of this study is to evaluate the antifibrotic effect of ring-type Sp1 decoy oligonucleotides (ODNs) through blocking the transcription of transforming growth factor (TGF)-β1 and its downstream target genes. In this experiment, the expression of TGF-β1, metalloproteinase (MMP)-13, and fibronectin was decreased in the group with the treatment of the ring-type Sp1 decoy ODNs. Also, α-smooth muscle actin positive bronchial lining cells and alveolar epithelial cells were observed, especially around the lesions of extracellular matrix (ECM) deposition. These findings provide evidences for the finding of pulmonary epithelial–mesenchymal transition (EMT) and the effectiveness of Sp1 transcription factor as a target for the gene therapy on lung fibrosis.