Hybridizing ability and nuclease resistance profile of backbone modified cationic phosphorothioate oligonucleotides

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• 作者：S.M. Abdur Rahman^a ; ^{rahman_du@yahoo.com} ; Takeshi Baba^b ; Tetsuya Kodama^b ; Md. Ariful Islam^a ; Satoshi Obika^b ; ^{obika@phs.osaka-u.ac.jp}
• 关键词：Phosphorothioate ; Backbone modification ; Aminoalkyl moiety ; UV melting experiment ; Nuclease resistance
• 刊名：Bioorganic and Medicinal Chemistry
• 出版年：2012
• 期刊代码：9_09680896
• 类别：ch
• 出版时间：1 July, 2012
• 卷：20
• 期：13
• 页码：4098-4102
• 文件大小：244 K

摘要

Various stereochemically pure cationic phosphorothioate oligonucleotides bearing aminoalkyl moieties were synthesized, and their duplex-forming ability against single-stranded DNA (ssDNA), single-stranded RNA (ssRNA) and triplex-forming ability against double-stranded DNA (dsDNA) were evaluated by UV melting experiments. The cationic Rp stereoisomers showed improved duplex-forming ability against ssDNA, triplex-forming ability against dsDNA and nuclease stability.