Viability, maturation and embryo development in vitro of immature porcine and ovine oocytes vitrified in different devices

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• 作者：Filiberto Ferná ; ndez-Reyez^a ; ^d ; ^{freyes@correo.xoc.uam.mx} ; Yvonne Ducolomb^b ; ^{duc@xanum.uam.mx} ; Salvador Romo^c ; ^{sromo_99@yahoo.com} ; Eduardo Casas^b ; ^{dino@xanum.uam.mx} ; Zayil Salazar^b ; ^{zayil.salazar@gmail.com} ; Miguel Betancourt^b ; ^{bet@xanum.uam.mx}
• 关键词：BES ; beveled edge open straw ; BSA ; bovine serum albumin ; COCs ; cumulus cell-oocyte-complexes ; DPBS ; Dulbecco&rsquo ; s phosphate buffered solution ; ED ; embryo development ; EG ; ethylene glycol ; FCS ; fetal calf serum ; GV ; germinal vesicle ; IVF ; in vitro ferti
• 刊名：Cryobiology
• 出版年：2012
• 期刊代码：166_00112240
• 类别：bio
• 出版时间：June, 2012
• 卷：64
• 期：3
• 页码：261-266
• 文件大小：195 K

摘要

This study was designed to evaluate the effects of vitrification on immature porcine and ovine oocytes, collected at a slaughterhouse, by performing vitrification in devices with different volumes. Viability was evaluated both before and after vitrification and maturation. Immediately after warming, the percentage of viable pig oocytes was 81%regardless the type of device, while in the control (after oocyte selection) was 95%. The viability of matured pig oocytes after warming, vitrified in beveled edge open straws (BES) was 6%, in small-open-pulled-straw (SOPS) was 17%and in cryotop was 4%, while the viability of the control group was 86%. The viability and maturation results were similar with all devices. Embryo development (ED) was observed in fresh porcine oocytes with 15%2-8 cell embryos, 7%morulae and 3%blastocysts, and non-embryo cleavage was observed in warmed oocytes. The viability of sheep oocytes immediately after warming averaged 90%in all devices, while that of the control (after oocyte selection) averaged 95%. The viability of warmed oocytes after maturation was: BES 21%, SOPS 30%, cryotop 21%and control group 86%; while maturation values were 11, 21, 34 and 70%, respectively. After vitrification, the highest ED was achieved with ovine oocytes vitrified in SOPS, with 17%morulae development and it was the only device in which blastocysts developed. A direct relationship was observed between viability and actin filament integrity in both species.