Different start-up strategies to enhance biohydrogen production from cheese whey in UASB reactors

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• 作者：Juliá ; n Carrillo-Reyes^a ; Lourdes B. Celis^b ; Felipe Alatriste-Mondragó ; n^a ; Elí ; as Razo-Flores^a ; ^{erazo@ipicyt.edu.mx}
• 关键词：Fermentation ; Hydraulic retention time ; Hydrogenotrophic ; Hydrogen ; Methane ; Organic loading rate
• 刊名：International Journal of Hydrogen Energy
• 出版年：2012
• 期刊代码：42_03603199
• 类别：ch
• 出版时间：April, 2012
• 卷：37
• 期：7
• 页码：5591-5601
• 文件大小：1036 K

摘要

The effect of different operational strategies and inoculum structure (granules and disaggregated granules) during the start-up of four up-flow anaerobic sludge blanket hydrogenogenic reactors was investigated. The more stable volumetric hydrogen production rates obtained were 0.38 and 0.36聽L H₂/L-d, in reactors operated with a constant organic loading rate (OLR) with both inoculum structures, whereas in reactors operated with an increasing OLR methane started to be produced earliest in time. Specific hydrogenogenic activity results proved that the disaggregated inoculum produced a more active biomass than the granular one, but not granule formation was evident. The methane hydrogenotrophic activity was the main limitation of the systems evaluated. In the reactors inoculated with disaggregated sludge the start-up strategy did not influence the bacterial DGGE fingerprint, in contrast to the reactors started-up with granular sludge; members of the Clostridium genus were always present. The results demonstrated that operational conditions during the start-up period are crucial for the production of hydrogenogenic biomass.