Effects of graded levels of dietary methionine hydroxy analogue on immune response and antioxidant status of immune organs in juvenile Jian carp (Cyprinus carpio var. Jian)

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• 作者：Sheng-Yao Kuang^a ; ^d ; Wei-Wei Xiao^a ; Lin Feng^a ; ^b ; ^c ; Yang Liu^a ; ^b ; ^c ; Jun Jiang^a ; ^b ; ^c ; Wei-Dan Jiang^a ; ^b ; ^c ; Kai Hu^a ; ^b ; ^c ; Shu-Hong Li^a ; Ling Tang^a ; ^d ; Xiao-Qiu Zhou^a ; ^b ; ^{xqzhouqq@tom.com} ; ^{zhouxq@sicau.edu.cn}
• 关键词：Cyprinus carpio var. Jian ; Methionine hydroxy analogue ; Immune response ; Antioxidant status
• 刊名：Fish & Shellfish Immunology
• 出版年：2012
• 期刊代码：115_10504648
• 类别：abs
• 出版时间：May, 2012
• 卷：32
• 期：5
• 页码：629-636
• 文件大小：296 K

摘要

Immune response and antioxidant status of immune organs in juvenile Jian carp (Cyprinus carpio var. Jian) fed graded levels of methionine hydroxy analogue (MHA) (0, 5.1, 7.6, 10.2, 12.7, 15.3聽g聽kg^鈭? diet) for 60 days were investigated. Results indicated that head kidney index, spleen index, red and white blood cell counts significantly increased with increasing MHA levels up to a point (P聽<聽0.05), whereupon decreased (P聽<聽0.05). Glutathione reductase activity in head kidney and spleen, anti-hydroxy radical and glutathione-S-transferase activities in spleen, catalase activity and GSH content in head kidney significantly increased by MHA supplement, while malondialdehyde content, anti-superoxide anion, superoxide dismutase, glutathione peroxidase activities in head kidney and spleen, protein carbonyl content and catalase activity in spleen, anti-hydroxy radical activity in head kidney significantly decreased by MHA supplement. However, protein carbonyl content and glutathione-S-transferase activity in head kidney, GSH content in spleen remained unaffected. After 60-day feeding trial, a challenge study was conducted by injection of Aeromonas hydrophila for 17 days. Results showed that survival rate, leukocytes phagocytic activity, lysozyme activity, acid phosphatase activity, total iron-binding capacity, haemagglutination titre, complement 3, 4 and immunoglobulin M contents significantly increased by optimal dietary MHA supplement (P聽<聽0.05). These data suggested that MHA affected antioxidant status of immune organs and promoted immune response in juvenile Jian carp.