Cloning and Sequencing of V Genes from Anti-Osteosarcoma Monoclonal Antibodies TP-1 and TP-3: Location of Lysine Residues and Implications for Radiolabeling

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• 作者：Olafsen ; Tove ; Bruland ; &Oslash ; yvind S. ; Zalutsky ; Michael R. ; Sandlie ; Inger
• 刊名：Nuclear Medicine and Biology
• 出版年：1995
• 期刊代码：43_09698051
• 类别：ph
• 出版时间：August, 1995
• 卷：22
• 期：6
• 页码：765-771
• 文件大小：593 K

摘要

Monoclonal antibodies TP-1 and TP-3 are of potential utility for the radioimmunodiagnosis of osteosarcoma in both human and canine patients. The V genes of these antibodies were cloned and sequenced and to facilitate radiolabeling of these proteins, the location of the lysine residues within these sequences have been determined. The V-domains of TP-1 contain a total of 12 lysines, 10 in the framework region and 2 in the CDR region, while the V-domains of TP-3 contain a total of 14 lysines, 11 in the framework region and 3 in the CDR regions. Using space-filling models, the availability of each lysine residue for radiolabeling, and potential interference with antigen binding was predicted.