Muscle-like nicotinic receptor accessory molecules in sensory hair cells of the inner ear
- 作者：Abdullah A. Osman ; Angela D. Schrader ; Aubrey J. Hawkes ; Omar Akil ; Adam Bergeron ; Lawrence R. Lustig ; Dwayne D. Simmons
- 关键词：Peripheral auditory system ; Synaptogenesis ; Cholinergic synapse ; Receptor assembly and clustering
- 刊名：Molecular and Cellular Neuroscience
- 出版年：2008
- 期刊代码：173_10447431
- 类别：bio
- 出版时间：June 2008
- 卷：38
- 期：2
- 页码：153-169
- 文件大小：3285 K
摘要
Nothing is known about the regulation of nicotinic acetylcholine receptors (nAChRs) in hair cells of the inner ear. MuSK, rapsyn and RIC-3 are accessory molecules associated with muscle and brain nAChR function. We demonstrate that these accessory molecules are expressed in the inner ear raising the possibility of a muscle-like mechanism for clustering and assembly of nAChRs in hair cells. We focused our investigations on rapsyn and RIC-3. Rapsyn interacts with the cytoplasmic loop of nAChR 
alpha" title="greek small letter alpha" border="0">9 subunits but not nAChR alpha" title="greek small letter alpha" border="0">10 subunits. Although rapsyn and RIC-3 increase nAChR alpha" title="greek small letter alpha" border="0">9 expression, rapsyn plays a greater role in receptor clustering while RIC-3 is important for acetylcholine-induced calcium responses. Our data suggest that RIC-3 facilitates receptor function, while rapsyn enhances receptor clustering at the cell surface.
alpha" title="greek small letter alpha" border="0">9 subunits but not nAChR alpha" title="greek small letter alpha" border="0">10 subunits. Although rapsyn and RIC-3 increase nAChR alpha" title="greek small letter alpha" border="0">9 expression, rapsyn plays a greater role in receptor clustering while RIC-3 is important for acetylcholine-induced calcium responses. Our data suggest that RIC-3 facilitates receptor function, while rapsyn enhances receptor clustering at the cell surface.