Protective effect of l-theanine on carbon tetrachloride-induced acute liver injury in mice

详细信息	查看全文 | 推荐本文 |

• 作者：Wei Jiang^a ; Min Gao^a ; Shuai Sun^a ; Aijing Bi^a ; Yinqiang Xin^b ; Xiaodong Han^c ; Liangbin Wang^a ; Zhimin Yin^b ; ^{yinzhimin@njnu.edu.cn} ; Lan Luo^a ; ^{lanluo@nju.edu.cn}
• 关键词：l-theanine ; Carbon tetrachloride ; Acute liver injury ; Oxidative stress ; Inflammatory response ; Apoptosis
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：1 June, 2012
• 卷：422
• 期：2
• 页码：344-350
• 文件大小：1295 K

摘要

We studied effects of l-theanine, a unique amino acid in tea, on carbon tetrachloride (CCl₄)-induced liver injury in mice. The mice were pre-treated orally with l-theanine (50, 100 or 200 mg/kg) once daily for seven days before CCl₄ (10 ml/kg of 0.2%CCl₄ solution in olive oil) injection. l-theanine dose-dependently suppressed the increase of serum activity of ALT and AST and bilirubin level as well as liver histopathological changes induced by CCl₄ in mice. l-theanine significantly prevented CCl₄-induced production of lipid peroxidation and decrease of hepatic GSH content and antioxidant enzymes activities. Our further studies demonstrated that l-theanine inhibited metabolic activation of CCl₄ through down-regulating cytochrome P450 2E1 (CYP2E1). As a consequence, l-theanine inhibited oxidative stress-mediated inflammatory response which included the increase of TNF-伪 and IL-1尾 in sera, and expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in livers. CCl₄-induced activation of apoptotic related proteins including caspase-3 and PARP in mouse livers was also prevented by l-theanine treatment. In summary, l-theanine protects mice against CCl₄-induced acute liver injury through inhibiting metabolic activation of CCl₄ and preventing CCl₄-induced reduction of anti-oxidant capacity in mouse livers to relieve inflammatory response and hepatocyte apoptosis.