Ontogeny of PAC1-R and VPAC1-R in the frog, Rana esculenta
- 作者：Monica Ciarlo ; Federica Bruzzone ; Cristiano Angelini ; Mauro Vallarino ; Hubert Vaudry
- 关键词：PACAP receptors ; Frog development ; Tissue expression ; In situ hybridization
- 刊名：Peptides
- 出版年：2007
- 期刊代码：59_01969781
- 类别：neu
- 出版时间：September 2007
- 卷：28
- 期：9
- 页码：1738-1745
- 文件大小：1864 K
摘要
The distribution of pituitary adenylate cyclase-activating polypeptide (PACAP) and PACAP receptors in the brain of amphibians has been previously described. In the present study, we have investigated the ontogeny of the selective PACAP receptor, PAC1-R, and the PACAP-vasoactive intestinal polypeptide (VIP) mutual receptor, VPAC1-R, in frog embryos by whole-mount in situ hybridization histochemistry. At stage 20, expression of PAC1-R and/or VPAC1-R mRNAs was detected in the brain, the auditory vesicles, the external gills, the buds of the lateral lines and the coelomatic cavity. At stage 25, PAC1-R and/or VPAC1-R mRNAs were observed in the buds of the orbital lateral line, the pancreas and heart. At stage 30, PAC1-R and VPAC1-R mRNAs were widely distributed in the telencephalon and diencephalon as well as in the bud of the lateral line, the heart and the pancreas. The anatomical distribution of PAC1-R and VPAC1-R mRNAs, although similar, did not totally overlap, indicating that PACAP and VIP may exert differential effects in frog during development.