Identification and expression analysis of IGFBP-1 gene from Japanese flounder (Paralichthys olivaceus)

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• 作者：Wanying Zhai¹ ; Junling Zhang¹ ; Zhiyi Shi ; ^{zyshi@shou.edu.cn} ; Yuanshuai Fu
• 关键词：Cloning ; Expression ; IGFBP-1 ; Paralichthys olivaceus ; Thyroid hormone
• 刊名：Comparative Biochemistry and Physiology ; Part B
• 出版年：2012
• 期刊代码：50_10964959
• 类别：bio
• 出版时间：April, 2012
• 卷：161
• 期：4
• 页码：413-420
• 文件大小：1753 K

摘要

Insulin-like growth factor binding protein-1 (IGFBP-1) plays an important role in IGF regulating vertebrate growth and development. In this study, we cloned IGFBP-1 cDNA from Japanese flounder (Paralichthys olivaceus) liver. The full-length cDNA is 1070 bp, including a 5鈥?untranslated region (UTR) of 69 bp, a 3鈥?UTR of 272 bp, and an open reading frame (ORF) of 729 bp encoding a polypeptide of 242 amino acids. Real-time quantitative PCR revealed that IGFBP-1 mRNA is mainly expressed in the liver, and a small amount of mRNAs was also found in other adult tissues. There are maternal transcripts of IGFBP-1 gene, and relatively low mRNA levels were observed in different embryonic stages. A higher level of IGFBP-1 mRNA was detected at 3 days post hatching (dph), and it got to the highest level at 29 dph (metamorphic climax), and finally brought back to a lower level at the end of metamorphosis. The expression of IGFBP-1 mRNA was greatly up-regulated in thyroid hormone (TH)-treated larvae, and declined after thiourea (TU) treatment. These results provide basic information for further studies on the role of IGF system in the P. olivaceus development and metamorphosis.