Potential of auxotrophic Edwardsiella tarda double-knockout mutant as a delivery vector for DNA vaccine in olive flounder (Paralichthys olivaceus)

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• 作者：Seung Hyuk Choi ; Ki Hong Kim ; ^{khkim@pknu.ac.kr}
• 关键词：Auxotrophic mutant ; Edwardsiella tarda ; DNA vaccine carrier
• 刊名：Veterinary Immunology and Immunopathology
• 出版年：2012
• 期刊代码：105_01652427
• 类别：abs
• 出版时间：15 February, 2012
• 卷：145
• 期：3-4
• 页码：625-630
• 文件大小：537 K

摘要

To evaluate potential of an auxotrophic Edwardsiella tarda mutant (螖alr 螖asd E. tarda) as a delivery vehicle for DNA vaccine in fish, olive flounder (Paralichthys olivaceus) were immunized with the E. tarda mutant harboring plasmids (pG02-ASD-CMV-eGFP) for eukaryotic expression of the enhanced green fluorescent protein (eGFP) gene through either intraperitoneal (i.p.) or oral route, and the expression of eGFP in the internal organs and generation of antibody against eGFP in fish were analyzed. In fish i.p. injected with 2 脳 10⁷ CFU/fish of 螖alr 螖asd E. tarda harboring pG02-ASD-CMV-eGFP, expression of eGFP was detected in liver, kidney, and spleen from 1 day to 28 days post-injection. In fish orally administered with 1 脳 10⁹ CFU/fish of the bacteria, the eGFP band was detected in liver, kidney, and spleen from 1 day to 14 days post-administration, whereas, in intestine, the band was detected only at 1 day post-administration. Either oral or i.p. immunization of olive flounder with recombinant E. tarda that carried eGFP-expressing eukaryotic plasmids was successful to induce humoral adaptive immunity against not only E. tarda that was used as a delivery vehicle but also eGFP that was used as the reporter protein of DNA vaccine, suggesting attenuated E. tarda-vectored DNA vaccine has a potential to be used as a combined vaccine against infectious diseases in fish.