Cholesterol Superlattice Modulates CA4P Release from Liposomes and CA4P Cytotoxicity on Mammary Cancer Cells

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• 作者：Berenice Venegas^&dagger ; ; Weiwei Zhu^&dagger ; ; Nicole  ; B. Haloupek^&dagger ; ; Janet Lee^&dagger ; ; Elizabeth Zellhart^&dagger ; ; Istvá ; n  ; P. Sugá ; r^&Dagger ; ; Mohammad  ; F. Kiani^&sect ; ; Parkson  ; Lee-Gau Chong^&dagger ; ; ^{pchong02@temple.edu}
• 关键词：CA4 ; combretastatin A4 ; CA4P ; combretastatin A4 disodium phosphate ; Cr ; critical cholesterol mole fractions theoretically predicted for maximal superlattice formation ; CR ; max ; the cholesterol mole fractions where the initial rate of drug release is maxima
• 刊名：Biophysical Journal
• 出版年：2012
• 期刊代码：P13_00063495
• 类别：bio
• 出版时间：2 May, 2012
• 卷：102
• 期：9
• 页码：2086-2094
• 文件大小：481 K

摘要

Liposomal drugs are a useful alternative to conventional drugs and hold great promise for targeted delivery in the treatment of many diseases. Most of the liposomal drugs on the market or under clinical trials include cholesterol as a membrane stabilizing agent. Here, we used liposomal CA4P, an antivascular drug, to demonstrate that cholesterol content can actually modulate the release and cytotoxicity of liposomal drugs in a delicate and predictable manner. We found that both the rate of the CA4P release from the interior aqueous compartment of the liposomes to the bulk aqueous phase and the extent of the drug's cytotoxicity undergo a biphasic variation, as large as 50%, with liposomal cholesterol content at the theoretically predicted C_r, e.g., 22.0, 22.2, 25.0, 33.3, 40.0, and 50.0聽mol%cholesterol for maximal superlattice formation. It appears that at C_r, CA4P can be released from the liposomes more readily than at non-C_r, probably due to the increased domain boundaries between superlattice and nonsuperlattice regions, which consequently results in increased cytotoxicity. The idea that the increased domain boundaries at C_r would facilitate the escape of molecules from membranes was further supported by the data of dehydroergosterol transfer from liposomes to M尾CD. These results together show that the functional importance of sterol superlattice formation in liposomes can be propagated to distal targeted cells and reveal a new, to our knowledge, mechanism for how sterol content and membrane lateral organization can control the release of entrapped or embedded molecules in membranes.