Parkin-co-regulated gene (PACRG) product interacts with tubulin and microtubules
- 作者：Takashi Ikeda
- 关键词：Axoneme ; Outer doublet microtubule ; Parkinson’ ; s disease ; Park2 ; Microtubule bundle ; Chlamydomonas
- 刊名：FEBS Letters
- 出版年：2008
- 期刊代码：70_00145793
- 类别：bio
- 出版时间：30 April 2008
- 卷：582
- 期：10
- 页码：1413-1418
- 文件大小：1140 K
摘要
Parkin-co-regulated gene (PACRG) is a gene that shares a bidirectional promoter with Parkinson’s disease-related Parkin/Park2 gene. Recently, the PACRG gene product was implicated in the function of flagella. However, its exact function remains unknown. Here, I assessed the interaction between PACRG and tubulin. Co-sedimentation experiments revealed that PACRG directly binds to microtubules and 
/β-tubulin heterodimers with high affinity. Microscopic studies showed that PACRG bundles microtubules and forms branched aggregates with unpolymerized tubulin dimers. The amino acid sequence of the microtubule-binding region of PACRG is highly conserved among various organisms, suggesting that tubulin binding is a basic property of PACRG.
/β-tubulin heterodimers with high affinity. Microscopic studies showed that PACRG bundles microtubules and forms branched aggregates with unpolymerized tubulin dimers. The amino acid sequence of the microtubule-binding region of PACRG is highly conserved among various organisms, suggesting that tubulin binding is a basic property of PACRG.