In vitro antioxidant activity of Parnassia wightiana W. extracts

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• 作者：Xin-Bao WEN^a ; Fang MIAO^a ; ^{miaofangmf@163.com} ; Le ZHOU^b ; Miao ZHANG^a ; Quan-Lei HE^a
• 关键词：Parnassia wightiana W ; Extracts ; Flavonoids ; Free radicals ; Antioxidant activity
• 刊名：Chinese Journal of Natural Medicines
• 出版年：2012
• 期刊代码：P72_18755364
• 类别：med
• 出版时间：May, 2012
• 卷：10
• 期：3
• 页码：190-195
• 文件大小：252 K

摘要

Aim

To investigate the total flavonoid contents and in vitro antioxidant activity of different extracts from Parnassia wightiana and their correlation.

Methods

50%ethanol (in water, V/V) extract from Parnassia wightiana W. was successively extracted by using 3 kinds of different solvents to provide chloroform extract (CE), ethyl acetate extract (EAE), n-butanol extract (BE) and water extract (WE). The content of flavonoids in each extract was determined using the NaNO₂鈭扐l(NO₃)₃-NaOH method. Different antioxidant assays (OH,路 O^{2鈭?/sup>,路 DPPH 路 scavenging activity, reducing power and metal chelating activity) were employed to evaluate the antioxidant activities of four extracts, using butylated hydroxytoluene (BHT) as a reference.}

Results

The total flavonoid content in EAE was the highest, followed BE, WE and CE. WE and BE showed better antioxidant activity than CE and EAE. Each extract showed concentration dependent antioxidant activity and a high correlation between content of flavonoids and antioxidant activity.

Conclusion

The extrat of Parnassia wightiana W. may be used as natural antioxidant.