Cell wall polysaccharides of Brassica campestris seed cake: isolation and structural features
- 作者：Ghosh ; Partha ; Ghosal ; Prodyot ; Thakur ; Swapnadip ; Lerouge ; Patrice ; Loutelier-Bourhis ; Corinne ; et. al.
- 关键词：Brassica campestris ; Seed cake ; Arabinan ; Arabinogalactan protein ; Rhamnogalacturonan I ; Xyloglucan ; Xylan
- 刊名：Carbohydrate Polymers
- 出版年：2004
- 期刊代码：14_01448617
- 类别：ch
- 出版时间：August 12, 2004
- 卷：57
- 期：1
- 页码：7-13
- 文件大小：205 K
摘要
Brassica campestris seed cake is an important source of water-soluble polysaccharides. The polysaccharides extracted with acid and dilute alkali represented 270 mg/g of defatted meal. These polymers, which have high amount of neutral sugars are arabinan, rhamnogalacturonan I and arabinogalactan proteins. These polymers have been analyzed by size exclusion chromatography. Two overlapping peaks have been found. Structural characterization of 4 M KOH-soluble hemicellulosic polysaccharides using specific enzyme hydrolysis, ion exchange chromatography (HPAEC) and matrix-assisted laser desorption ionization-time of flight mass spectroscopy showed that mustard meal xyloglucan is of XXXG type and contained XXXG, XXFG, XXLG and XLFG (named according to Fry et al. [Physiol. Plant. 89 (1993) 1]) as the major building sub-units. Hydrolysis with endo-β-(1→4)-d-xylanase and analysis of the xylan derived oligosaccharides showed the presence of xylose (27%), xylobiose (26%) and acidic xylan fragments containing 4-O-methyl-d-glucuronic acid residue (47%).