Effect of injected sample amount on the shape of chromatographic peaks under condition of linear partition isotherm
- 作者：Vezzani ; S. ; Moretti ; P. ; Peri ; R. ; Castello ; G.
- 关键词：Capillary columns ; Inlet pressure ; Sample concentration ; Peak shape ; Peak symmetry ; Partition isotherm ; Adsorption ; Mathematical models
- 刊名：Journal of Chromatography A
- 出版年：2005
- 期刊代码：47_00219673
- 类别：ch
- 出版时间：February 18, 2005
- 卷：1065
- 期：2
- 页码：231-237
- 文件大小：214 K
摘要
In a previous paper a model function was tested in order to approximate the peak shape obtained on non-polar column by injecting different compounds. The simulation of the symmetrical or non-symmetrical shape of gas chromatographic peaks was satisfactory. In this paper, the influence of the amount of injected substance was investigated at different values of inlet pressure and carrier gas velocity, in order to evaluate the relative contribution to the total peak area and shape of the symmetrical distribution due to partition phenomena and of the non-symmetrical and tailing distribution due to adsorption–desorption kinetics. The effect of the molecular mass and of the chain length of compounds belonging to the homologous series of 1-alcohols and n-alkanes on the adsorption phenomena was evaluated.