Efficient differentiation of CD14+ monocytic cells into endothelial cells on degradable biomaterials
- 作者：Guido Krenning ; Patricia Y.W. Dankers ; Danijela Jovanovic ; Marja J.A. van Luyn ; Martin C. Harmsen
- 关键词：Cell culture ; Endothelial cell ; Monotype ; Polycaprolactone ; Polyurethane ; RGD peptide
- 刊名：Biomaterials
- 出版年：2007
- 期刊代码：7_01429612
- 类别：ms
- 出版时间：March 2007
- 卷：28
- 期：8
- 页码：1470-1479
- 文件大小：1325 K
摘要
Vascular tissue engineering aims at creating self-renewing, anti-thrombogenic, vascular grafts, which can be based on endothelial progenitor cells (EPC). EPC harbor essential features such as plasticity and longevity. Unfortunately, the archetype CD34+ EPC is rare in peripheral blood. Monocytes, i.e. CD14+ cells also have the ability to differentiate into endothelial-like cells and are by far more abundant in peripheral blood than are CD34+ EPC. Therefore, CD14+ cells would seem appropriate candidates for tissue engineering of small-diameter blood vessels. In this study, we investigated the differentiation of CD14+ cells on three biodegradable biomaterials under angiogenic conditions. Morphological analyses, gene transcript analyses, endothelial marker (i.e. VE-Cadherin and eNOS) and macrophage marker (i.e. CD68 and CD163) expression analyses, revealed that a small fraction (15–25%) of cultured CD14+ cells differentiated into macrophages after 21 days of culture. The majority of CD14+ cells (>75%) differentiated into endothelial-like cells (ELC) on all biomaterials used. The expression of endothelial markers was similar to their expression on HUVEC. Since CD14+ cells are present in high numbers in adult peripheral blood, easy to isolate and because they easily differentiate into ELC on biomaterials, we conclude that CD14+ cells are a suitable cell source for progenitor-based vascular tissue engineering.