Non-lethal heat shock protects gnotobiotic Artemia franciscana larvae against virulent Vibrios

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• 作者：Yeong Yik Sung ; Els J.M. Van Damme ; Patrick Sorgeloos ; Peter Bossier
• 关键词：Non-lethal heat-shock ; Heat shock proteins ; Artemia franciscana ; Immune response ; Challenge test ; Vibrio campbellii ; Vibrio proteolyticus
• 刊名：Fish & Shellfish Immunology
• 出版年：2007
• 期刊代码：115_10504648
• 类别：abs
• 出版时间：April 2007
• 卷：22
• 期：4
• 页码：318-326
• 文件大小：215 K

摘要

Brine shrimp Artemia were exposed under gnotobiotic conditions to a non-lethal heat shock (NLHS) from 28 to 32, 37 and 40 °C. Different recovery periods (2, 6, 12 and 24 h) and different heat-exposure times (15, 30, 45 and 60 min) were tested. After these NLHS, Artemia was subsequently challenged with Vibrio. Challenge tests were performed in stressed and unstressed nauplii at concentrations of 10⁷ cells ml⁻¹ of pathogenic bacteria, Vibrio campbellii and Vibrio proteolyticus. A NLHS with an optimal treatment of 37 °C for 30 min and a subsequent 6 h recovery period resulted in a cross-protection against pathogenic Vibrio. A 100%increase in the larval survival (P < 0.05) was observed. We have also demonstrated by Western blot that a NLHS increases the expression of HSP-70 in heat-shocked (HS) treated animals. This report is the first to reveal a cross protection of a NLHS against deleterious bacterial challenges in living crustaceans. The putative role of heat shock proteins (HSPs) in this process is discussed.