Treatment with haloperidol or clozapine causes changes in dopamine receptors but not adenylate cyclase or protein kinase C in the rat forebrain

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• 作者：Wan ; David C. ; Dean ; Brian ; Pavey ; Geoffrey ; Copolov ; David L.
• 关键词：haloperidol ; clozapine ; dopamine receptors ; adenylate cyclase ; protein kinase C ; forebrain
• 刊名：Life Sciences
• 出版年：1996
• 期刊代码：62_00243205
• 类别：imm
• 出版时间：November 1, 1996
• 卷：59
• 期：23
• 页码：2001-2008
• 文件大小：528 K

摘要

The effect of treating rats with daily injections of haloperidol (1 mg/kg/day) or clozapine (20 mg/kg/day) for four weeks on second messengers and dopamine receptors was studied. The binding of [³H]forskolin to adenylate cyclase (AC), [³H]phorbol 12,13-dibutyrate (PDBu) to protein kinase C (PKC), [³H]SCH23390 binding to the dopamine D₁ (DA-D₁) receptor and [³H]spiperone binding to the dopamine D₂ (DA-D₂) receptor were measured using quantitative autoradiography. The density of AC was greatest in the caudate-putamen, nucleus accumbens and olfactory tubercle, a distribution resembling that of DA-D₁ receptor. The distribution of PKC was relatively homogeneous in the forebrain. Neither haloperidol nor clozapine administration significantly altered the levels of AC or PKC in the caudate-putamen. By contrast treatment with haloperidol, but not clozapine, significantly increased the density of DA-D₂ receptors in the caudate-putamen without affecting the density of DA-D₁ receptors. By contrast, both haloperidol and clozapine increased the density of DA-D₁ receptors in the olfactory tubercle.