In-vitro DNA binding and cleavage studies with pBR322 of N,N-Bis(3尾-acetoxy-5伪-cholest-6-yl-idene)hydrazine

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• 作者：Zishan Tabassum^a ; Mohd Muddassir^b ; Othman Sulaiman^a ; Farukh Arjmand^b ; ^{farukh_arjmand@yahoo.co.in}
• 关键词：UV&ndash ; vis titration ; Fluorescence study ; pBR322 DNA ; DNA cleavage
• 刊名：Journal of Luminescence
• 出版年：2012
• 期刊代码：54_00222313
• 类别：ch
• 出版时间：August, 2012
• 卷：132
• 期：8
• 页码：2178-2181
• 文件大小：323 K

摘要

The DNA binding studies of the triterpenoid derivative, cholesterol, N,N-Bis(3尾-acetoxy-5伪-cholest-6-yl-idene)hydrazine (L) with CT DNA were carried out by employing different optical methods viz, UV-vis and fluorescence spectroscopy. The ligand binds to DNA through hydrophobic interaction with K_b value found to be 4.7脳10³ M^鈭?. These observations have been validated also by fluorescence spectroscopy. (L) exhibits a remarkable DNA cleavage activity with pBR322 DNA in the presence of different activators and the DNA is probably cleaved by an other than oxidative mechanism, possibly by a discernable hydrolytic pathway. In the presence of major and minor groove binding agents, (L) prefers major groove binding of the DNA.