In t
his study, teleostean fis
h Leporinus obtusidens (piava) were exposed to different concentrations of Roundup, a glyp
hosate (acid equivalent)
herbicide: 0 (control), 3, 6, 10, and 20 mg/L for 96
h (s
hort-term). Acetylc
holinesterase (AC
hE) activity was verified in brain and muscle tissues. Metabolic p
arameters in t
he liver and muscle (lactate, glycogen, glucose, protein, and ammonia), as well as some
hematological p
arameters, were determined. Unexposed fis
h ex
hibited significantly
hig
her brain AC
hE activity w
hen compared to t
hat of t
he muscle (
href="/science?_ob=MathURL&_method=retrieve&_udi=B6WDM-4H4T60S-1&_mathId=mml7&_user=10&_cdi=6770&_rdoc=13&_handle=V-WA-A-W-AY-MsSAYVA-UUA-U-AAZCWUEZBY-AAZWDEUVBY-VAEZCABC-AY-U&_acct=C000050221&_version=1&_userid=10&md5=00d7d227aa406e3796251772885d9c1f" title="Click to view the MathML source">P<0.05) (13.8±0.76 and 6.1±1.31 μmol/min/g protein, respectively). Results indicated t
hat AC
hE activity significantly decreased in t
he brain of fis
h exposed to all glyp
hosate concentrations tested, but in t
he muscle t
his p
arameter was not altered. In addition, fis
h exposed to all glyp
hosate concentrations s
howed a significant increase in
hepatic glycogen and glucose, but a significant reduction in muscle glycogen and glucose. Lactate and protein of fis
h exposed to all glyp
hosate concentrations presented a significant decrease in t
he liver, but did not c
hange significantly in t
he muscle. Levels of ammonia in bot
h tissues increase in fis
h at all glyp
hosate concentrations. Exposure to t
his
herbicide produced a decrease in all
hematological p
arameters tested. T
hese results indicate t
hat AC
hE activity as well as metabolic and
hematological p
arameters may be good early indicators of
herbicide contamination in
L. obtusidens.