Enhancement of cypermethrin degradation by a coculture of Bacillus cereus ZH-3 and Streptomyces aureus HP-S-01

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• 作者：Shaohua Chen¹ ; Jianjun Luo¹ ; Meiying Hu ; ^{humy@scau.edu.cn} ; Kaiping Lai ; Peng Geng ; Huasheng Huang
• 关键词：Degradation ; Cypermethrin ; Microbial consortium ; Metabolites ; Coculture
• 刊名：Bioresource Technology
• 出版年：2012
• 期刊代码：11_09608524
• 类别：ce
• 出版时间：April, 2012
• 卷：110
• 期：Complete
• 页码：97-104
• 文件大小：577 K

摘要

Degradation of cypermethrin was significantly enhanced in a coculture of Bacillus cereus ZH-3 and Streptomyces aureus HP-S-01. In the pure culture, longer half-lives (t_1/2 = 32.6-43.0 h) of cypermethrin were observed, as compared to the mixed cocultures (t_1/2 = 13.0 h). The optimal degradation conditions were determined to be 28.2 掳C and pH 7.5 based on response surface methodology (RSM). Under these conditions, the mixed cultures completely metabolized cypermethrin (50 mg L^鈭?) within 72 h. Analysis of degradation products of cypermethrin indicated that the microbial consortium converted cypermethrin to 伪-hydroxy-3-phenoxy-benzeneacetonitrile, 3-phenoxybenzaldehyde and 4-phenoxyphenyl-2,2-dimethyl-propiophenone, and subsequently transformed these compounds with a maximum specific degradation rate (q_max), half-saturation constant (K_s) and inhibition constant (K_i) of 0.1051 h^鈭?, 31.2289 mg L^鈭? and 220.5752 mg L^鈭?, respectively. This is the first report of a proposed pathway of degradation of cypermethrin by hydrolysis of ester linkage and oxidization of 3-phenoxybenzyl in a coculture. Finally, this coculture is the first described mixed microbial consortium capable of metabolizing cypermethrin.