Liquid chromatography study of degradation and metabolism of pentachloronitrobenzene by four soil micromycetes

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• 作者：Torres ; Rocio Mora ; Grosset ; Catherine ; Steiman ; Ré ; gine ; Alary ; Josette
• 关键词：pentachloronitrobenzene ; micromycetes ; biodegradation ; metabolites ; liquid chromatography
• 刊名：Chemosphere
• 出版年：1996
• 期刊代码：15_00456535
• 类别：env
• 出版时间：August, 1996
• 卷：33
• 期：4
• 页码：683-692
• 文件大小：517 K

摘要

The pentachloronitrobenzene (PCNB) biodegradation by four soil micromycetes, Mucor racemosus,Sporothrix cyanescens, Paecilomyces farinosus and Pithomyces chartarum was studied after 24 h and 120 h. Quantitation of PCNB and identification of its metabolites were monitored by LC analysis of the culture media on a 5 μm Adsorbosphere HS C₁₈ column with methanol/water (93:7) pH 3.5 (1.0 ml/min) and UV detection at 301 nm. The extraction process of PCNB and its metabolites was performed on an Extrelut® cartridge with ethyl acetate as eluent. Strains were able to degrade PCNB with high efficiency. Five metabolites were identified : pentachloroaniline, tetrachloroaniline, pentachlorothiophenol, pentachlorothioanisole and pentachloroanisole. Based on our findings, three metabolic pathways were retained.