Determination of aflatoxin B₁ levels in deep-red ground pepper (isot) using immunoaffinity column combined with ELISA

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• 作者：Mustafa Ardic ; Yakup Karakaya ; Mustafa Atasever ; Hisamettin Durmaz
• 关键词：Aflatoxin B₁ ; Red ground pepper ; Deep-red ground pepper ; Spices
• 刊名：Food and Chemical Toxicology
• 出版年：2008
• 期刊代码：20_02786915
• 类别：pha
• 出版时间：May 2008
• 卷：46
• 期：5
• 页码：1596-1599
• 文件大小：102 K

摘要

Deep-red ground pepper, a variety of red ground pepper, is a special spices belonging to Sanliurfa and consumed both in Sanliurfa and other provinces of Turkey. The aim of this study was to determine the aflatoxin B₁ (AFB₁) levels of deep-red ground pepper. For this purpose, 75 samples of deep-red ground pepper (isot) marketed in Sanliurfa (Turkey) were purchased from bazaars and herbal shops. The occurrence and concentration range of AFB₁ in the samples were investigated by microtitre plate Enzyme Linked Immunosorbent Assay (ELISA) method using immunoaffinity columns. Seventy-two of the 75 ground deep-red pepper samples (96%) contained AFB₁ in the range of 0.11–24.7 μg/kg. Eleven (14.7%) samples were above the regulatory limits used in the European Union and in Turkey. More precaution should be taken on hygiene controls in order to prevent microbiological and chemical hazards.