Involvement of perivascular nerves and transient receptor potential vanilloid 1 (TRPV1) in vascular responses to histamine in rat mesenteric resistance arteries

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• 作者：Honghua Jin^a ; Pengyuan Sun^a ; ^b ; Shingo Takatori^a ; Toshihiro Koyama^c ; Yoshito Zamami^d ; Panot Tangsucharit^a ; Yoshihisa Kitamura^c ; Hiromu Kawasaki^a ; ^{kawasaki@pheasant.pharm.okayama-u.ac.jp}
• 关键词：Histamine ; Vasoconstriction ; Vasodilation ; Perivascular nerve ; Transient receptor potential vanilloid 1 ; Rat mesenteric resistance artery
• 刊名：European Journal of Pharmacology
• 出版年：2012
• 期刊代码：24_00142999
• 类别：neu
• 出版时间：5 April, 2012
• 卷：680
• 期：1-3
• 页码：73-80
• 文件大小：891 K

摘要

A previous report showed that histamine in denuded mesenteric vascular beds produced a triphasic response; an initial small histamine H₂ receptor-mediated vasodilation, a transient histamine H₁ receptor-mediated vasoconstriction, and finally a long-lasting vasodilation. We further investigated the vascular effect of histamine in mesenteric preparations without an endothelium to clarify the possible involvement of perivascular nerves. Male Wistar rat mesenteric vascular beds without an endothelium were perfused with Krebs solution containing methoxamine to produce active tone and lafutidine to block histamine H₂ receptor-mediated vasodilation. Histamine (1-100 渭M) was perfused for 1 min and perfusion pressure was measured with a pressure transducer. Histamine caused a biphasic vascular response; initial vasoconstriction followed vasodilation. Tetrodotoxin (a neurotoxin, 1 渭M) and procaine (a local anesthetic, 100 渭M) significantly inhibited the vasoconstriction and vasodilation. Ruthenium red (a transient receptor potential vanilloid 1 (TRPV1) antagonist, 1 渭M) also significantly inhibited both phases of the response. Pretreatment with capsaicin (a depletor of calcitonin gene-related peptide (CGRP)-containing nerves, 5 渭M) significantly inhibited the vasodilation without affecting the initial vasoconstriction. Both indomethacin (a cyclooxygenase inhibitor, 0.5 渭M) and seratrodast (a thromboxane A₂ receptor antagonist, 0.1 渭M) abolished the histamine-induced vasoconstriction and subsequent vasodilation. These results suggest that histamine-induced vasoconstriction and long-lasting vasodilation are mediated by activation of TRPV1 on capsaicin-sensitive and capsaicin-insensitive nerves. They also suggest that perivascular nerves and prostanoids, probably thromboxane A₂, are responsible for the vascular response to histamine.