Cultivar and anatomical analysis of corolla enlargement of petunia (Petunia hybrida Vilm.) by cytokinin application
- 作者：Takaaki Nishijima ; Hideari Miyaki ; Kazuya Sasaki ; Tatsuo Okazawa
- 关键词：Anatomy ; Corolla size ; Cultivar difference ; Cytokinins ; Petunia (Petunia hybrida Vilm.)
- 刊名：Scientia Horticulturae
- 出版年：2006
- 期刊代码：93_03044238
- 类别：abs
- 出版时间：4 December 2006
- 卷：111
- 期：1
- 页码：49-55
- 文件大小：845 K
摘要
We found that the corolla of petunia (Petunia hybrida Vilm.) could be conspicuously enlarged by the separate application of three cytokinins: forchlorfenuron (CPPU), N6-benzylaminopurine (BA), and zeatin. To obtain the same enlargement as that achieved by CPPU, approximately 30 and 900 times the concentration of BA and zeatin, respectively, were required. CPPU at 3.2 μmol/L increased the limb area of the corollas of 15 cultivars to between 1.3 and 2.4 times (1.8 times on average) the size of the control area. The increase was negatively correlated (R = 0.58) with the “genetic” limb area (i.e., that of the untreated plant). The enlargement of the corolla caused by cytokinin application was mainly attributed to an increase in cell number in most cultivars. This increase resulted from a high rate of cell proliferation and from prolongation of the cell proliferation phase during corolla development. This anatomical change caused by cytokinin application was similar to the anatomical difference among cultivars because genetic differences in limb area resulted mainly from differences in cell number.