A high yield robotic synthesis of 9-(4-[¹⁸F]-fluoro-3-hydroxymethylbutyl)guanine ([¹⁸F]FHBG) and 9-[(3-[¹⁸F]fluoro-1-hydroxy-2-propoxy)methyl]guanine([¹⁸F]

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• 作者：C.W. Chang ; M. Lin ; S.Y. Wu ; C.H. Hsieh ; R.S. Liu ; S.J. Wang ; K.L. Huang ; C.H. Chen ; H.E. Wang
• 关键词：[¹⁸F]FHBG ; [¹⁸F]FHPG ; HSV1-tk ; Gene imaging ; Robotic system
• 刊名：Applied Radiation and Isotopes
• 出版年：2007
• 期刊代码：6_09698043
• 类别：ce
• 出版时间：January 2007
• 卷：65
• 期：1
• 页码：57-63
• 文件大小：254 K

摘要

The aim of this study was to develop an automated synthesis of 9-(4-[¹⁸F]-fluoro-3-hydroxymethylbutyl)guanine ([¹⁸F]FHBG) and 9-[(3-[¹⁸F]fluoro-1-hydroxy-2-propoxy)methyl]guanine ([¹⁸F]FHPG) using a Scanditronix Anatech RB III robotic system. [¹⁸F]HF was produced via ¹⁸O(p, n)¹⁸F using a Scanditronix MC17F cyclotron. On average, a typical run produced [¹⁸F]FHBG and [¹⁸F]FHPG with an uncorrected radiochemical yield of 19%and 16%, respectively, at end of synthesis (EOS) from irradiation of 95%enriched [¹⁸O]water. The total synthesis time was 80 min. The retention time of [¹⁸F]FHBG and [¹⁸F]FHPG (the radio-peak) was 3.9 and 4.0 min, respectively, which was consistent with the [¹⁹F]FHBG and [¹⁹F]FHPG ultraviolet peak. The radiochemical purity was greater than 97%. A robotic, automated method for [¹⁸F]FHBG and [¹⁸F]FHPG radiosynthesis is therefore feasible. The radiation burden for the operator can be reduced as much as possible. Sufficient radioactivities of [¹⁸F]FHBG and [¹⁸F]FHPG could be obtained for non-invasive monitoring the expression of transfected gene in vivo with positron emission tomography (PET).