Endogenous semicarbazide-sensitive amine oxidase (SSAO) inhibitor increases 1-methyl-4-phenylpyridinium ion (MPP+)-induced dopamine efflux by immobilization stress in rat striatum
- 作者：Toshio Obata
- 刊名：International Journal of Developmental Neuroscience
- 出版年：2006
- 期刊代码：28_07365748
- 类别：neu
- 出版时间：August 2006
- 卷：24
- 期：5
- 页码：343-347
- 文件大小：120 K
摘要
The present study examined whether or not immobilization stress (IMMO)-inducible semicarbazide-sensitive amine oxidase (SSAO) inhibitor by separated gel filtration from 105,000 g supernate in rat brain cytosol contribute to the dopamine (DA) efflux by 1-methyl-4-phenylpyridinium ion (MPP+) in the rat striatum. The isoelectric point (pI) value of this inhibitor was determined by isoelectric focusing (IEF)-gel electrophoresis to about 3.8. The application of IMMO-induced SSAO inhibitor (3 μg) by IEF-gel electrophoresis increased DA efflux by MPP+ in rat striatum. These results suggest that IMMO-inducible endogenous SSAO inhibitor enhances DA efflux by MPP+.