Tumor and Non-Tumor Liver Angiogenesis Is Traced and Evaluated by Hepatic Arterial Ultrasound in Murine Models

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• 作者：Clarisse Eveno^&lowast ; ; ^&dagger ; ; Carole Le Henaff^&Dagger ; ; Raphaë ; lle Audollent^&lowast ; ; Philippe Soyer^§ ; ; Aurore Rampanou^&lowast ; ; Judith Nemeth^鈥?/sup> ; Jean-Philippe Brouland^鈥?/sup> ; Evelyne Dupuy^&lowast ; ; Marc Pocard^&lowast ; ; ^&dagger ; ; Philippe Bonnin^&lowast ; ; ^¶ ; ; ^{philippe.bonnin@lrb.aphp.fr}
• 关键词：Ultrasonography ; Liver ; Metastasis ; Angiogenesis ; Mice ; Liver regeneration
• 刊名：Ultrasound in Medicine and Biology
• 出版年：2012
• 期刊代码：82_03015629
• 类别：ph
• 出版时间：July, 2012
• 卷：38
• 期：7
• 页码：1195-1204
• 文件大小：1053 K

摘要

We studied the relationships between hepatic and mesenteric mean blood-flow velocities (mBFVs) measured by ultrasound imaging and (1) downstream tumor angiogenesis during liver metastasis induced by spleen injection of LS174 human colon cells overexpressing the antiangiogenic Netrin4 (LS174-NT4) or not (LS174-WT) and (2) downstream normal angiogenesis during hepatic regeneration after 50%hepatectomy. Liver volume and mBFVs were measured before and after surgery, at day 30 in the first model and at days 2, 7 and 16 in the second model. LS174-NT-4 vs. LS174-WT mice presented fewer metastases (25%vs. 90%, p < 0.001) and decreased hepatic mBFVs (16.5 卤 0.8 vs. 21.8 卤 1.4 cm s^鈭?, p < 0.01), without difference in mesenteric mBFVs. After partial hepatectomy, hepatic and mesenteric mBFVs increased at day 7, from 12.4 卤 1.7 and 11.8 卤 2.6 to 19.1 卤 1.8 and 17.5 卤 2.4 cm s^鈭?, respectively, (p < 0.01) then returned to baseline as liver volume. Duplex Doppler ultrasonography reliably assesses normal or tumor angiogenesis and may provide follow-up functional evaluation.