Regulation of lipid biosynthesis by phosphatidylinositol-specific phospholipase C through the transcriptional repression of upstream activating sequence inositol containing genes

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• 作者：Sunny D. Rupwate^a ; Preeti S. Rupwate^a ; Ram Rajasekharan^a ; ^b ; ^{lipid@biochem.iisc.ernet.in}
• 关键词：PKC ; protein kinase C ; DAG ; diacylglycerol ; CDP-DAG ; cytidinediphosphate diacylglycerol ; Glu-6-P ; glucose 6-phosphate ; Gro-3-P ; glycerol-3-phosphate ; Ino-3-P ; inositol-3-phosphate ; IP3 ; inositol trisphosphate ; IP4 ; inositol tetrakisphosphate ; IP5 ; inosito
• 出版年：2012
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：21 May, 2012
• 卷：586
• 期：10
• 页码：1555-1560
• 文件大小：554 K

摘要

The regulation of phospholipid biosynthesis in Saccharomyces cerevisiae through cis-acting upstream activating sequence inositol (UAS_ino) and trans-acting elements, such as the INO2-INO4 complex and OPI1 by inositol supplementation in growth is thoroughly studied. In this study, we provide evidence for the regulation of lipid biosynthesis by phosphatidylinositol-specific phospholipase C (PLC) through UAS_ino and the trans-acting elements. Gene expression analysis and radiolabelling experiments demonstrated that the overexpression of rice PLC in yeast cells altered phospholipid biosynthesis at the levels of transcriptional and enzyme activity. This is the first report implicating PLC in the direct regulation of lipid biosynthesis.