Kinetics of n-butyl benzyl phthalate degradation by a pure bacterial culture from the mangrove sediment
- 作者：Xiang-Rong Xu ; Hua-Bin Li ; Ji-Dong Gu ; Xiao-Yan Li
- 关键词：n-Butyl benzyl phthalate ; Biodegradation ; Kinetics ; Endocrine-disruptor ; Pseudomonas fluorescens B-1
- 刊名：Journal of Hazardous Materials
- 出版年：2007
- 期刊代码：50_03043894
- 类别：ce
- 出版时间：9 February 2007
- 卷：140
- 期：1-2
- 页码：194-199
- 文件大小：380 K
摘要
n-Butyl benzyl phthalate (BBP) is an endocrine-disrupting chemical. Biodegradation of BBP by a bacterium, Pseudomonas fluorescens B-1, isolated from the mangrove sediment, was investigated. The microorganism can utilize BBP as the sole carbon and energy source, where concentrations of BBP disappeared within 6 days under shake culture conditions. Effects of BBP concentration, pH, temperature, and salinity on BBP biodegradation were studied, respectively. The process of BBP biodegradation was monitored by reversed-phase high-performance liquid chromatography with ultra-violet detection after solid-phase extraction. The biodegradation of BBP could be fitted to a first-order kinetic model. The major metabolites of BBP degradation were identified as mono-butyl phthalate, mono-benzyl phthalate, phthalic acid and benzoic acid by gas chromatography–mass spectrometry, and a preliminary metabolic pathway of BBP was proposed.